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• o • • • • .. . . o 00 • 10 INTRODUCTION .. o 0 • o go. 0 e • • o A. Statement of the Problem • • • • o Nerve Tissue Culture Techniques. o 10 Tissue Culture as a Research Nethod .. 2. Early Developments • o 3. Recent Advances • o C. Structura.l Characteristics of Cultured. N~:!:-<.Te Tissue • Nerve Cells • o • .. • 0 .. • '0 2. Neuroglia 0 o • • • HyeUn Formation • • . D. Functional Characteristics of Cultured Nerve Tissue • Biochemical Parameters • 2. E1ectrophysiological Parameters • E. Comments on Nerve Tissue Cultures • F .. Radiation Effects on Nerve Tissue • o Dose Dependent Radiation Effects .. . 2. Age Dependent Radiation Effects .. . .. II.. MATERIALS AND METItODS • . .. .. .. . A. Tissue Culture Methods .. .. .. 1. Biological Material 20 Medium • • • Incubation .. • . .. 4. Examination of Cultures .. o • Factors Influencing Culture Success .. B. Histologj.cal Nethods • . .. . . . ~ vi

Effects of ionizing radiations on myelin formation in rat brain cultures