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Pharmaceuticals in the environment have attracted significant attention in recent years. The method here details the analysis of carbamazepine (CBZ), oxcarbazepine (OXC) and metabolites carbamazepine-epoxide (CBZ-epoxide), 10,11-dihydro-10-hydroxycarbamazepine (CBZ-10OH), and 10,11-dihydro-10,11-hydroxycarbamazepine (CBZ-DiOH), from sampling sites within a wastewater treatment plant (WWTP). Samples (500 mL) were prepared using Oasis HLB solid phase extraction cartridges and analyzed by liquid chromatography tandem mass spectrometry and quantified using an internal standard (CBZ-D10). The limit of quantitation ranged from 0.01 to 0.1 ng on-column. Composite samples were fortified with a mix of parent pharmaceuticals and metabolites for method validation. Reasonable recoveries were assessed for CBZ, CBZ-epoxide, and CBZ-10-OH, and CBZ-DiOH (> 70%) and low recoveries (44%) for OXC were observed. Concentrations of metabolites in effluent are detailed here for the first time for U.S.-based

California GAMA Special Study: Analysis of Carbamazepine, Oxcarbazepine and Metabolites as Wastewater Tracers in Water Resource Studies