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The bone alkaline phosphatase has clinical importance in pathological orthopedic conditions as a marker of the osteoblastic activity, especially in malignant bone tumors and therapeutic monitoring of osteoporosis and other diseases. The reaction of Roy quantitative and qualitative technique of thermal inactivation was applied to obtain bone alkaline phosphatase isoenzyme of male and female donkeys, without defined breed (SRD) aged from seven to 14 months, subjected to the same diet, divided into two groups, composed of five animals each, being the group 1 (G1) kept in confinement and the Group 2 (G2) in traction activity. Blood samples were collected in eight times with an interval of 90 days, during 21 months. The percentage of thermostable isoenzyme was 35 for the G1 and 32 for the G2. It was concluded that the measurements of bone alkaline phosphatase by the thermal inactivation method showed no changes when compared among the groups, demonstrating that the activity of animal traction exerted by the donkeys do not interfere in bone metabolism of these animals.

Bone alkaline phosphatase in donkeys obtained by the thermal inactivation method