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1) The glycerinated muscle fiber develops a tension of about 0.8g/mm2 by 0.5M Thoulet's reagent.2) The shortening of the glycerinated muscle fiber by Thoulet's reagent is prevented by K, Mg and Ca ions coexisted in Thoulet's reagent. However, even with1.9M KCl, the grade of shortening decreases less than 10%.3) Salyrgan bears no marked influence on the Thoulet's reagent shortening of the glycerinated muscle fiber.4) The Thoulet's reagent shortening of the glycerinated muscle fiber increases in degree wtih rise of temperature in the range of 0° to 60°C.5) Even in low concentration (10-4M), Thoulet's reagent shows a marked inhibition on the ATP contraction of the glycerinated muscle fiber.6) Glycerinated tendon is shortened to about 10-20% of its original length by Thoulet's reagent.7) Thoulet's reagent shortening of the glycerinated muscle fiber bears a striking resemblance to the shortening caused by CuCl2 and that by high temperature, but differs in many points from ATP contracti

EFFECTS OF THOULET'S REAGENT ON THE GLYCERINATED MUSCLE FIBER