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Protein solutions were irradiated with doses (1055-105r) of gamma-ray from Co60.1. The peaks of gamma-and beta-globulin begin to approach as the dose increases and make only one peak which lies in the middle of two fractions.2. When the concentration of oxygen present in protein solution is very low, irradiation causes no denaturation of protein.3. The oxygen concentration in solution shows a marked decrease after irradiation as compared with that before irradiation.4. The mechanism of protein denaturation produced by irradiation and the role of oxygen were discussed.

PROTEIN DENATURATION BY IONIZING RADIATION AND ROLE OF OXYGEN