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An airtight and watertight closed chamber for intravital microscopy of the rat mesentery was developed. It incorporates an oxygen microelectrode and a thermistor for monitoring the local environment around the mesentery, as well as a heater for controlling the chamber temperature. This chamber provides an alternative to the superfusion method for intravital microscopy of the mesenteric microcirculation under circumstances where well-defined experimental conditions are required.

A Closed Chamber for Intravital Microscopy of the Rat Mesentery under Controlled Oxygen Tension and Temperature.