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Using a direct cellular micropuncture technique with double-barreled ion-selective microelectrodes, we investigated the effect of dibutyryl-cyclic AMP (db-cAMP) on the membrane potential and the transport of Na+, K+, and H+ in doubly-perfused bullfrog proximal tubules. The peritubular membrane potential difference (EM) and the intracellular K+, Na+ activities ((K)i, (Na)i) or intracellular and luminal pH were monitored continuously after peritubular administration of db-cAMP (10(-3)-10(-4)M).

The effect of cAMP on ion transport in the proximal tubular cells in bullfrog kidney.