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Determination of Hüfner's factor and simultaneous measurement of met- and carboxyhemoglobin were performed in 31 human, 16 canine, and 2 murine blood samples. Both measured and corrected values of Hüfner's factor were lower than the theoretical value of 1.39, suggesting the possibility of the presence of unidentifiable inactive hemoglobin(s) in blood.

Determination of Huefner's factor and inactive hemoglobins in human, canine, and murine blood.