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A new method of horseradish peroxidase (HRP) neurohistochemistry using o-dianisidine (OD) as the chromogen was described. In labelling neurones of the hypoglossal nucleus in rats and cats, the new OD method was as sensitive as Mesulam's method using tetramethyl benzidine (TMB) as the chromogen.

An o-dianisidine method of horseradish peroxidase neurohistochemistry.