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We present a test of our novel methodology for noninvasive quantitative characterization of human skin in vivo involving a customary pressure-cuff test in healthy volunteers at different cuff pressures. Our experimental approach combines time-resolved measurements of mid-infrared emission from sample surface after exposure to a short light pulse and diffuse reflectance spectroscopy in visible part of the spectrum. Both data sets are fitted simultaneously with predictions of a dedicated numerical model of light transport in a four-layer skin model (i.e., inverse Monte Carlo). This approach allows assessment of, e.g., blood contents and oxygenation levels in papillary and reticular dermis.

Monitoring of Hemodynamics in Human Skin Using Pulsed Photothermal Radiometry and Optical Spectroscopy