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The present study aimed to investigate the effect of combined salt (three salinity levels of 0.0M, 0.1M and 0.2M NaCl), heat (20 and 30C) and drought (without drought and drought stress by polyethylene glycol (PEG) at 8000 ppm) stresses on seed germination, seed and seedlings vigor traits of three lentil cultivars (Giza 9, Giza 51 and Sinai 1). Increasing temperature from 20C to 30C significantly decreased most studied characters. Both salinity levels significantly decreased all traits comparing with the control treatment (0 salt NaCl). All traits were more sensitive to 0.2M.than 0.1M.NaCl. Effects of drought were insignificant on shoot length, root length, seedling vigor index and root/shoot ratio. Drought significantly decreased seed germination by 5.6% and seed vigor by 4.87%. Cultivar Giza 9 was more tolerant to drought than the other two cultivars. Gel electrophoresis (SDS-PAGE) analysis for lentil cultivars under salt stress and drought stress at two different temperatures produced new protein heat shock protein (HSP) presented under 30C and was absent under control treatment. The applied laboratory technique is valid to be used for screening large number of lentil accessions for their performance under studied stresses as a rapid technique before screening them for such stresses under field condition.

EFFECT OF DROUGHTAND COMBINED SALT AND HEAT STRESSES ON GERMINATION AND SEEDLING GROWTH PATTERNS OF LENTIL