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This study was carried out in a laboratory of Plant Physiology, Faculty of Agriculture, Al-Azhar University, Nasr city Cairo, Egypt during 2013 – 2016 A.D to comparison between Rosa hybrida L. cultivars (Anna and Gold strike cvs.) reported that Anna cv. have the shortlongevity vase life (early senescence) and Gold strike cv. which have long-longevity (late senescence). The effect of exogenous sucrose (Suc), ethanol (Eth), ethanol + sucrose (Eth+Suc), salicylic acid (SA), 5-sulfosalycilic acid (5-SSA), silver thiosulphate (STS) and benzyladenine (BA) on the longevity of vase life for two cut rose flowers (Anna and gold strike cvs.) was investigated. Some physiological and biochemical traits such as water relations (water uptake, water loss and relative fresh weight), stability membrane, electrolyte leakage, chlorophyll a,b and carotenoids, lipid peroxidation, thiobarbituric acid reactive substances (TBARS), hydrogen peroxide (H2O2), peroxidase (POD), superoxide dismutase enzyme (SOD) activity and endogenous hormones i.e. indole acetic acid (IAA), cytokinin (CKs), gibberellic acid (GA3) and abscisic acid (ABA) were measured. Results reported that, the vase life evidently all treatments were better treated, 1mM SA, 6% Eth, 6% Eth + 2% Suc recorded vase life valued 7,6 and 6 days, respectively with Anna cv. compared with their control (4 days), while Gold strike cv. with same better treated recorded 12,11 and 11 days, respectively when compared with control (8 days). Results reported that ethanol 6% + 2% sucrose and salicylic acid at 1 mM reduced lipid peroxidation and consequently prolonged membrane integrity, antioxidant enzymes (POD and SOD) activity observed was dramatically in two cultivars. While, water relations (water uptake, water loss and relative fresh weight) was better for the best treatments compared to control. Also pigments of photosynthetic and membrane stability were more stable with the best treatments compared with untreated plants. Furthermore, the levels of CKs, IAA and GA3 were increased in treated plants, while, ABA decreased in both cut ‘Anna and Gold strike’ rose flowers compared untreated plants. Overall, the results suggest that SA and combination of ethanol + sucrose increase vase life by improving the antioxidant system and endogenous growth hormones and reducing oxidative stress damages during rose flower senescence. In general, Gold strike cv. was more responsive than Anna cv. to proposed transactions. On the other hand, the lowest coefficients were STS, 5-SSA and BA treatments.

SENESCENCE OF ROSE FLOWERS 2- REGULATION AGING AND PROLONG THEIR VASE LIFE