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Light microscopy, scanning and transmission electron microscopy were used to study the morphology and theultrastructure of the pollen grains of Withania somnifera (L.) Dunal. Light microscopic examination revealed that thepollen grain is tri- or tetrazonocoplate, approximately as long as broad measuring 29-μm. Scanning electron microscopic observation showed that surface sculpture is scarbate granulate. Ultrathin sections as examined by transmission electron microscope showed that the pollen contained numerous starch grains, liped drops, endoplasmic reticulum and vesicles of dicotysomes. Two layers of pollen wall were also distinguished; the outer wall (exine divided into ektexine and enexine as well as an inner layer (intine). The nutritive values of Withania pollen were discussed. The importance of studying the ultrastructure of pollen grains as a new tool in plant taxonomy was alsodiscussed.

Ultrastructure of the pollen grains of Withania somnifera (L.) Dunal (Solanaceae), A study from Saudi Arabia