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A Bacillus cereus strain was isolated from Egyptian soil area after a rapid screening in Menufia governorate to select heparinase hyper-producing microbes. The strain gave an enzyme production of 159.6 U/ml after 192 hours of incubation on PYH medium. Chicken slaughter byproducts were used as a supplement for production of pharmaceutical heparinase. The environmental waste was used with different concentrations (1%, 5%, 10%, 15% and 20%) to optimize the process of bioremediation. Enzyme production was gave maximum when using a waste percentage of 15%. Optimum production of the extracellular enzyme was obtained at pH 9.5 and 37 o C. Produced enzyme was determined both biochemically and electrochemically in the microbial liquid culture to evaluate the efficiency of the polyion detection method in enzyme detection and both methods gave a similar results. Extracellular heparinase enzyme was purified with gel filtration. Specific activity of the purified enzyme reached 152.12 (U/mg).

Production and Purification of Heparin Degrading Enzyme from Bacillus Cereus upon Biodegradation of Domestic Chicken Slaughter Byproducts