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A total of 60 Nile tilapia fish Oreochromis niloticus were collected from River Nile (Damietta branch): Weesh El-Hagar village during late summer. An experimental infection was applied on apparently healthy Nile tilapia (three equal groups) by intramuscular injection of 0.1 ml of 2.4X107 CFU/ml of well identified A. hydrophila suspension isolated from freshwater crayfish P. clarkii, while, the third group was injected 0.1 ml of sterile buffered saline . The clinical picture, morbidity and mortality rates were recorded for 7 days. The results showed that all injected tilapia died by the end of experiment with a mortality peak on the 3rd day, while the morbidity started to appear by the 2nd day (10%). The histopathological alterations were recorded and discussed. This study suggests an epidemiological significance of Aeromonas hydrophila and the role of crayfish in its transmission.

Role of Freshwater Crayfish Procambarus clarkii in Transmission of Motile Aeromonas Septicemia to Nile Tilapia Oreochromis n (one mark)