English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background: Diabetic nephropathy is a serious microvascular complication of type 2 diabetes mellitus that can progress slowly to end-stage renal disease that can be fatal. It is characterized by highly specific, slowly progressing histopathological changes. As the standard biomarkers lack the adequate sensitivity, novel biomarkers are needed for the early detection and risk stratification of diabetic nephropathy. Aim of Study: To investigate the histopathological changes occurring in diabetic nephropathy, to study the associated changes of two novel biomarkers; Liver Fatty Acid Binding Protein (L-FABP) and Kidney Injury Molecule (KIM), to correlate their gene expression with the degree of histopathological changes and to compare their sensitivity and degree of correlation against the standard biomarkers, notably blood urea and serum creatinine. Material and Methods: 24 experimental male rats were divided into two groups, a control group, and a diabetic nephropathy group. Type 2 diabetes mellitus was induced by High Fat Diet (HFD) followed by Streptozotocin (STZ) intraperitoneal injection. The presence of diabetes was confirmed by fasting plasma glucose & insulin levels, and the induction of diabetic nephropathy was confirmed by the presence of albuminuria. Histological examination was performed for all experimental rats using hematoxylin and eosin stain (H & E) staining as well as Periodic Acid Schiff (PAS) stain. These results were expressed as the mean optical density of the PAS-positive reaction. The gene expression for L-FABP and KIM were measured by Polymerase Chain Reaction (PCR) reverse transcriptase method and the levels of blood urea and serum creatinine, as standard biomarkers were measured. Statistical correlation analysis was performed by Pearson's correlation method to investigate the correlation of their gene expression and the histopathological changes. Correspondence to: Dr. Mohamed Mansour Khalifa, E-Mail: m_mmk2050@yahoo.com

the medical journal of cairo university/the medical journal of cairo university

Comparative Study of Novel Biomarkers of Kidney Injury; Kidney Injury Molecule and Liver Type Fatty Acid Binding Protein in Experimentally Induced Diabetic Nephropathy in Rats, and their Correlation with Renal Histopathological Changes