English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background: Zinc is involved in many biochemical functions. Over 300 enzymes require zinc for their activation and nearly 2000 transcription factors require zinc for gene expression. Zinc is also an effective antioxidant and anti-inflammatory agent. Nutritional deficiency of zinc may affect nearly 2 billion subjects in the developing world. Aim of Study: Because of the prevalence of malnutrition throughout the world this study was carried out to detect the possible effects of zinc-deficient pregnant albino rats on the retinal development of their embryos and offspring. Material and Methods: Female rats after mating were randomly divided into 2 equal groups: One control group received single intraperitoneal injection of distilled water on day 9 of gestation and one experimental group received intraperitoneal injections of 1.10 phenanthroline (zinc chelator purchased from Sigma Company), in a single dose of 30 mg/kg on day 9 of gestation. From the embryos and offspring of both control and treated mothers (at the following prenatal gestational days: 13, 15, 17, 19 and the following postnatal days: (newborn, 7 days, 15 days and 21 days), retinal specimens were processed for Haematoxylin & Eosin staining technique and immunohistochemical study using caspase-3 antibody was used to detect apoptosis in the postnatal age groups. Results: The light microscopic study of the 13 days zinc deficient embryo retina showed apparent shrinkage of the lens vesicle and the optic cup was well formed but it appeared to be shrunken. The retinal pigment epithelium (RPE) of 15 th , 17t h and 19t h day old zinc-deficient embryos consistently contained apoptotic cells with vacuolated cytoplasm and rarified nuclei. The neuroblastic layer showed apparent decrease in thickness and less mitotic features. The ganglion cell layer revealed numerous deeply stained pyknotic nuclei in the 19th gestational day. Retinae of the newborn, 7 days, 15 days, 21 days-old zinc deficient albino rats showed that the (RPE) contained some vacuolated cells with pyknotic nuclei. The organization of the retina was altered in zinc deficient rats. In addition, apparent reduction in the total retinal thickness and degenerative changes in some layers Correspondence to: Dr. Noha A. Rashed, E-Mail: noharashed2006@gmail.com. were observed. By the immunohistochemical study, there was intense positive caspase-3 reaction in all layers of the treated rat retina. Conclusion: This study revealed that zinc has a role in the developing rat retina and its deficiency causes cytoarchitectural and maturational changes in the retina of the albino rats. These histological changes may impair the visual functions of the retina; so it is recommended that further investigations should be carried out to determine the relevancy of deprivation of zinc as an etiologic factor in the development of retinal degeneration.

the medical journal of cairo university/the medical journal of cairo university

The Effect of Zinc-Deficient Pregnant Albino Rats on the Retinal Development of their Embryos and Offspring: Light Microscopic and Immunohistochemical Study