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The goal of the present study was to investigate the effect of cattle theileriosis on general health condition and some haematological and biochemical parameters specially Ascorbic acid and vitamin E as a model for studying the blood oxidative status in parasitized cattle . Atotal number 140 caws were subjected to the study their age ranged from 6 months to over 5 years belonged to some private farms in different localities at Kalubeia governorate ,all animals were examined clinically showed 46suspected cases (30.7%)which had signs of theileria infection and the laboratory parasitological examination confirmed the infection with theileria annulata the rest of the animals were proved healthy and used as control .The affected caws had high body temperature (40-41C),enlarged superficial lymphnodes ,anorexia ,congestion of mucous membranes and increased respiratory rates .Inadvances cases of the disease signs of anemia ,general weakness in addition to presence of ticks on animal body . The study cleared that animals at age of 6 months to one year have a higher rate of infection (33.3%)followed by the age of 1-3 years (30%),3-5 years (23.3%) and finally over 5 years (8%). Hematological investigation of the affected animals proved presence of anemia represented by significant decrease of total erythrocytic count ,haemoglobin concentration and packed cell volume .The leukogram showed that there was leukocytosis as aresult of neutrophilia ,lymphocytosis and monocytosis . The result of evaluation study of chain –breaking antioxidants vitamin E and ascorbic acid revealed significant decrease of these vitamins in diseased animals compaired with control . Concerning with biochemical changes the infected animals showed significant decrease in total protein, albumin and glucose level .Activities of serum transaminases (AST,ALT)total bilirubin ,urea and creatinine showed significant increase . Hematological And Biochemical Studies On... Amira M.M. Metwally & Soad S.Belah Kafr El-Sheikh Vet.Med.J. Vol. 1 No.1 (2003) 39 INTRODUCTION Tropical theileriosis is tick borne disease of cattle caused by Thileria annulata infection appears to be endemic in Egypt consider one of the major health problems of live stock (Shkap et.,al 2003 and Aktas et.,al 2004) .The disease is amajor programs in many parts of the middle east and Asia where about 200 millon cattle are said to be at risk of infection (Bakheit and Latif 2002). Bovine theileriosis clinically manifested by marked fever ,swelling of superficial lymph nodes ,inappetance ,tachycardia ,dyspenia and general weakness (Omer et.,al 2003) the mains symptoms of this disease is anemia (Abdel-Rahman et.,al 1989).Studies of (Forsyth et., al 1999) had represented anew view on the destructive effect on the parasite on the host organs and tissue Antioxidant vitamins such as E,Cand A protect the cell from damage by free oxygen radical generated as aresult of parasitism (Medzyavichyus et.,al1989) and (Smith 1989). Vitamin C status of ruminants may be important in relation to their health and disease. Ascorbic acid play an important role as an antioxidant (Kanter 1998). It has also been suggested to be of value in alleviating both physiological and pathological stress (Hemingway1991). More over ,the interaction between antioxidant vitamin Cand E was investigated and it was reported that there is asparing effects of vitamin C on vitamin .E (Miller et., al 1993) . The present study aimed to focus light on this disease ,its clinical signs ,age susceptibility ,the changes of some hematological blood biochemical component and alteration of antioxidant agents vitamin Eand ascorbic acid in response to cattle theileriosis. Hematological And Biochemical Studies On... Amira M.M. Metwally & Soad S.Belah Kafr El-Sheikh Vet.Med.J. Vol. 1 No.1 (2003) 40 MATERIALS AND METHODS 1-Animals: The present study was carried out on 140 caws aged from 6 months to over 5 years old collected from different private farms in different localities at Kalubeia governemate during summer season .The animals were clinically examined for signs of health and disease ,special care to body temprature ,corneal opacity and wasting according to the method described by (Radostits et., al 2002). The animals divided into two groups ,the first consisted of 94 apparantly healthy animals (free from blood parasite after blood examination )and kept as control group .The second consisted of 46 animals suffered from signs of the disease during blood examination of these animals ,besides presence of ticks on their body . 2Samples : Samples were taken from animals as follow: aThin blood smears were individually prepared from superfacial ear vein ,fixed and stained with Giemsa stain for detection of blood parasites microscopically according to (Feldman et.,al 2000). bAspirated smears were also taken from superfacial enlarged lymph nodes and examined according to (Marquardt et.,al 2000). cTwo blood samples were collected from jugular vein from each examined animals in dry sterile centrifuge tubes the first blood sample was collected with anticoagulant (Sodium salt of EDTA) and used for hematological studies according to (Feldman et.,al 2000), the second sample was collected in plain centrifuge tube for serum biochemical investigation .Serum total proteins (Peters1968) albumin (Drupt 1974) serum globulins were calculated ,glucose Hematological And Biochemical Studies On... Amira M.M. Metwally & Soad S.Belah Kafr El-Sheikh Vet.Med.J. Vol. 1 No.1 (2003) 41 (Trinder 1969) total bilirubin (Weigl 1975), aspartate aminotranceferace (AST) alanine amino transferase (ALT)activities (Reitman and Frankel 1957) ,urea (Fossati et.,al 1980) as well as creatinine (Seeling and Wust 1969),blood serum levels of ascorbic acid and vitamin E were determined according to (Omaye et.,al 1979)and Martin 1964) respectively dAll data were subjected to statistical analysis by stander T-test according to (Tamhane and Dunlop 2000). RESULTS 1-Clinical finding: Diseased animals were found invested with ticks in preneal ,flank and neck regions .Clinical examination revealed rise in body temperature ,enlargement of superfacial lymph nodes ,weakness ,anorexia nasal discharge ,lacrimation which sometimes reach corneal opacity and tachycardia . 2-aboratory finding: Theileria infected cattles were identified by examination of blood smear which revealed the presence of intracellular signet ring theileria species parasites .The results of the study dealing with parasitological and blood biochemical parameters were illustrated in tables as follow. Table (1): The Prevalence of T. annulated infection among Cattle’s in Kalubeia governarate. Examined animals No No of infected Infection % Apparently healthy 94 0 0 % Suspected disease 46 43 93.4

HEMATOLOGICAL AND BIOCHEMICAL STUDIES ON CATTLE THEILERIOSIS WITH SPECIAL REFERENCE TO ITS EFFECT ON SOME ANTIOXIDANT AGENTS