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The pituitary adenohypophysis was obtained from thirty buffaloes-cows, their age's ranges from one month to12 years. Sections of adenohy- pophysis tissues were immuno- stained for α, βa, and βb subunits of inhibin hormone. Positive immuno- staining specific for the α subunits of inhibin were detected in the cells of the follicles of the adenohypophysis in all ages. Moreover, immunostain- ing specific for the inhibin βa subu- nits were strong positive at one month, weak positive at 4.5, 8 and 12 years and were negative at 8 months and 1.5 years. However, the immunostaining specific for the in- hibin βb subunits were positive at one and 8 months, 8 and 12 years and weak positive at 4.5 years and negative at 1.5 year. RT-PCR anal- ysis revealed that both α and βb subunits are expressed in all ages except at 1.5 years old animals while βa subunit is only expressed at young age.

Immunohistochemical, cellular localization and expression of inhibin hormone in the buffalo (Bubalus bubalis) adenohypophysis at different ages