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In this study we evaluated the control potential effect of five doses of the entomopathogenic nematode, Steinernema carpocapsae (All) and Heterorhabditis bcteriophora (HP88) as a biocontrol agent against potato tuber moth, Phthorimaeao perculella under laboratory conditions. Using five concentrations of each nematode species (500, 1000, 1500, 2000, and 2500 infective juveniles, IJs) that inoculated in two stages(larvae and pupae) of the potato tuber moth. Mortality percentages were recorded along 5 days for all doses and calculated for each insect stage at different dose of entomopathogenic nematode. The larval mortality percentages was 90% and 100% at dose 2000IJs/ 10 individualsat the second and the third days,respectively, by S. carpocapsae and H. bcteriophora. The highest mortality percentages obtained from the dose 2500 IJs/10individuals by using H. bcteriophora 50%, 90% and 100% at the first, second and third days, respectively. At dose 2500 and 2000 IJsapplied for pupal stage not exhibit more than 40% and 50% by using S. carpocapsae and 50% and 70 % by using H. bcteriophora, respectively mortality percentages at the fifth day of the experiment. Our work indicates that P. operculella larvae were achieving high mortality percentages and it can applying entomopathogenic nematodes as biocontrol agents against P. operculella.

journal of plant protection and pathology /journal of plant protection and pathology

Evaluating the Role of Entomopathogenic Nematodes for the Biological Control of the Potato Tuber Moth, Phthorimaeao perculella under Laboratory Conditions.