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Four promising natural plants previously proved to prolong the activity of Spodoptera littoralis (Boisd.) nuclepolyhedrovirus (SpliNPV) suspension under Egyptian field conditions, examined alone or in combination with virus in six mixtures. Screening results were based on bioassay. Different parameters were investigated to prove the value of protection, % of mortality, reduction %, LIT50, Potency and Original Activity Remaining (OAR).DPPH assay was investigated for all treatments. The obtained results showed that, virus mixed with cacao + green coffee treatment gave the highest LIT50 (203.474 hours) while scored only (20.172 hours) with virus alone treatment. Original activity remaining indicated that SpliNPV mixed with both cacao + green coffee treatment preserved 100% of its activity for five days while it gave only 1.04 % for SpliNPV alone treatment and gave 41.06, 31.17, 17.65 and 42.56 with cacao, green coffee, moringa and both cacao + moringa additives; respectively five days post investigation. This investigation suggests that mixing natural derived antioxidants provides good protection to baculovirus at Egyptian sunny conditions.

journal of plant protection and pathology /journal of plant protection and pathology

Mixture of Natural Antioxidants can Improve Spodoptera littoralis (Boisd.) Nucleopolyhedrovirus, (Baculoviridae) Stabilty Under Egyptian Field Conditions