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The present study was conducted to utilize the gamma irradiation to alleviate the deterioration of Egyptian clover seed quality caused by artificial aging. Egyptian clover seeds  (c.v. Serw1)  were artificially aged at 41Co and 100% relative humidity for 3 and 5 days and then they were irradiated  with gamma ray doses (0, 50, 100, 150 and 200 Gy) of radioactive cobalt (60 CO) gamma rays on seed viability as measured by different laboratory tests including Standard germination (SG), and Electrical Conductivity (EC), Tetrazolium test (TZ), evaluation of seedling vigor indicators, and in addition to filed emergence (FE) under field conditions. The results revealed that artificial ageing increased electrical conductivity of seed leaching; but it decreased the number of viable seeds colored by tetrazolium Formozan (TZ). The germination percentage of aged seeds at standard conditions was also reduced and consequently seedling vigor traits, field emergence, relative field emergence showed the same trend. Exposing aged clover seed to Gamma rays (150 Gy) compared with other treatments improved germination traits, field emergence, relative field emergence. However, at higher dose of 200 Gy stress was evident and significant decreases in all parameters were observed when the seeds were aged for 5 days. Generally, the harmful effects of artificial aging (or poor storage conditions) on clover (c.v. Serw 1) seed performance under laboratory and filed conditions can alleviated by gamma irradiation dose of (150 Gy). Further investigation is needed including high doses of gamma radiation more than 200 Gy and seeds of various crop species stored naturally at controlled and open air storage, in order to confirm the results and find out the right recommendation.

Improved Performance of Egyptian Clover Seed Using Gamma Ray.