English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Fasciola gigantica causes a worldwide waterborne/foodborne zoonotic disease in which humans are incidental hosts. Fascioliasis has a major impact on human health and its control mainly depends on triclabendazole (TCBZ). Unfortunately, the effectiveness of this drug is decreased because of indiscriminate use resulting in development of resistance. Therefore, the search for another effective anthelmintic is now compulsory. This work aimed to evaluate the in vitro anthelmintic effects of auranofin (a thioredoxin reductase inhibitor) on adult F. gigantica in comparison with the drug of choice; TCBZ. This study involved in vitro petri dish incubation of seventy-five adult F. gigantica worms of nearly equal size with the tested drugs and classified into five groups (fifteen worms each) as follows; G1 served as a control group, G2 was exposed to TCBZ (20μL/mL), G3, G4, & G5 were exposed to auranofin (3, 5, & 10μg/mL, respectively). All adult worms were incubated and observed for three hours and subjected to motility and egg hatchability assays, histopathological and ultrastructural studies, glutathione-S-transferase and superoxide dismutase assay, and cathepsin-L gene expression analysis. Auranofin in all concentrations significantly decreased adult motility and egg hatchability. It induced histopathological and ultrastructural deformities including apoptosis. Auranofin in higher concentrations significantly suppressed the activity of the detoxifying enzyme; glutathione-S-transferase, and significantly stimulated superoxide dismutase enzyme activity reflecting the oxidative stress. At all concentrations, it suppressed the expression of the cathepsin-L gene responsible for Fasciola invasive function.

IN VITRO EVALUATION OF THIOREDOXIN REDUCTASE INHIBITOR (AURANOFIN) ACTIVITY IN COMPARISON WITH TRICLABENDAZOLE ON ADULT FASCIOLA GIGANTICA