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The goal of this study was to assess effect of three Tris-extender types, of egg yolk (E1), soy lecithin (E2), and coconut milk (E3), on sperm characteristics of Holstein bull semen at freezing stages. Semen was collected and pooled on each collection day. Semen should have good mass motility ≥70%. The pooled semen was divided into three divisions; E1: extended by E1: Tris-citric with 15% egg yolk; E2 and E3: diluted with 0.5% soy-lecithin and 15% coconut milk at a rate of 1:15, respectively. Semen with each extender was diluted and cooled at 5C for 4 hours, as equilibration period. Then, semen was packaged in 0.25 ml French straws and frozen in Liquid nitrogen (-196 °C) for one month. Results showed that E2 showed higher positive impact on sperm characteristics than E1 and E3 after dilution and equilibration, and thawing in terms of livability, plasma membrane integrity, motility, intact acrosome of spermatozoa, but the differences were not significant. In post-thawed semen -E2 was showed higher (P<0.05) progressive motility, and livability than other semen extenders. Also, E2 showed (P<0.05) higher sperm plasma membrane integrity, and intact acrosome, but the differences were not significant. The percentage of sperm abnormalities was similar in all extenders. In conclusion, this study recommended that 0.5% soy bean lecithin can be used instead of 15% egg yolk or 15% coconut milk for dilution and cryopreservation of bull semen.

Sperm Characteristics of Holstein Bull Semen Cryopreserve by using Three Various Types of Extenders