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This study aimed to evaluate the effect of exogenous multiple insulin injections on ovarian and estrus activity, and pregnancy rate in repeat breeder Friesian cows. Cows (n=12) in heat (Day 0) were divided into three groups. Cows were subcutaneous injected with 0.2 IU insulin/kg LBW on day 9 (G1), 8, 9 and 10 (G2), or 7, 8, 9, 10 and 11 (G3) of the normal estrous cycle. In G1, G2 and G3, cows were i.m. injected with 3 ml PGF2α on day 12. All treated cows in the next heat within 48-72 h postPGF2α treatment were artificially inseminated with proven fertile semen. Based on overall mean, results showed that follicular number was the highest in G1 and the lowest in G3 (P<0.05). Follicular diameter was higher (P<0.05) in G3 than in G1. Largest follicles diameter was not affected. Diameter of CL was higher (P<0.05) in G2 and G3 than in G1. Serum P4 concentration was higher (P<0.05) in G2 and G3 than in G1. Serum P4 concentration was highest in G3. Pregnancy rate was higher (P<0.05) in G3 (75%,) than in G1 and G3 (50%). The administration of multiple injections of insulin at days 7, 8, 9, 10 and 11, followed by 3 ml PGF2α on day 12 from initiation of normal estrous cycle improved ovarian and estrous activity, and pregnancy rate of repeat breeders Friesian cows.

journal of animal and poultry production

Multiple Insulin Injections to Improve Ovarian Activity and Pregnancy of Repeat Breeder Friesian Cows حقنات الأنسولین المتعددة لتحسین النشاط المبیضى والحمل فى الأبقار الفریزیان المتکررة التلقیح