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A total number of 1260 fertile eggs were randomly divided into seven treatments to investigate effect of in ovo-injection of ascorbic, folic acids and their combination at 14 day of incubation on hatchability and subsequent growth performance of broiler chicks. The experimental design was as follow: 1Eggs were without injection. 2Eggs were injected with 0.1ml distiller water/ egg. 3Eggs were injected with 0.1ml solution containing 6 μg ascorbic acid (AA) /egg. 4Eggs were injected with 0.1ml solution containing 75 μg folic acid (FA) /egg. 5Eggs were injected with 0.1ml solution containing 150 μg FA /egg. 6Eggs were injected with 0.1ml solution containing 6 μg AA + 75μg FA/egg. 7Eggs were injected with 0.1ml solution containing 6 μg AA+150μg FA /egg. Hatchability % was improved due to injection of AA6/egg and with combination between AA6 and FA150/egg as compared to the control group. Also, in ovo injected with AA6, FA75, FA150 and AA6+FA75 recorded the best value of live body weight compared to the control group at 28 days of age. In ovo injection of AA, FA and their combination significantly decreased triglycerides levels in serum blood. Also, the level of HDL significantly improved due to in-ovo injected with AA6 and FA75. Therefor, it could be mentioned that in-ovo injection of 6μg AA/egg and 6μg AA+150μg FA/egg in fertile eggs at 14 day of incubation can be applied to improve the hatchability and subsequent growth performance from 1 to 28 day of age.

Effect of In-Ovo Injection of Ascorbic, Folic Acids and their Combination on Hachability and Subsequent Growth Performance of Broiler Chicks