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A total number of 300 Sinai laying hens and 30 cocks, 27 wksold were used. The laying hens and cocks were weighed, and randomly divided into ten experimental groups with three replicates for each to investigate effects of layer dietary supplementation different levels of organic manganese (Mn-Met), selenium (Se-Met) and their mixture on productive and reproductive performance, internal egg quality, Mn and Se retention during the laying period from 27 – 43 weeks of age. The layer diets contained 1.5% soybean oil as a source of essential fatty acids and it contained gradually levels of organic Mn (20, 40, and 60 mg organic Mn /kg diet), organic Se (0.1, 0.2 and 0.3 mg organic Se /kg diet) and their mixture (20+0.1, 40+0.2 and 60+0.3 mg /kg diet (organic Mn + organic Se), where the diets premix of treatments 2,3 and 4 without Mn, 5,6 and 7 without Se and 8, 9 and 10 without Mn and Se compared to the control diet which contained mineral Mn and Se. Results showed that hens fed diets supplemented with 40mg organic Mn + 0.2 mg organic Se/ Kg diet, 60 mg organic Mn and 0.2 mg organic Se / Kg diet returned to occupy the first position in egg number as compared to the control diet by about 5.88, 5.49 and 4.66% respectively. The superiority of egg mass produced from hens fed diets supplemented with 0.2mg organic Se, 40mg organic Mn + 0.2mg organic Se and 40mg organic Mn/Kg diet. The best feed conversion resulted from the diet supplemented 0.2 mg organic Se, 40 mg organic Mn+ 0.2mg organic Se /kg diet and 60 mg organic Mn / kg diet. All dietary treatments recorded the lowest values in terms of content fresh yolk of total cholesterol. The diet contained 0.1mg organic Se and 40mg organic Mn /kg diet produced eggs with higher total antioxidant as compared with the control diet. In stored yolks, hens fed diets supplemented with 0.1, 0.2 mg organic selenium and 20mg organic Mn + 0.1mg organic Se / kg diet produced eggs with lower cholesterol concentration content. The fertility % of eggs from hens fed diets supplemented with 0.2 mg organic Se and 40 mg organic Mn / Kg diet increased compared to the control died. The higher value of scientific hatchability % occurred as a result of the diets supplemented with40 mg organic Mn and 40 mg organic Mn+0.2 organic Se /Kg diet / Kg diet than the control diet. All dietary treatments had significant higher selenium retention than control diet. The diet supplemented with 0.2 mg organic Se diet and 60 mg organic Mn /Kg diet resulted in decrease in total serum cholesterol compared to the control diet. The diet with organic Mn and Se together at the medium dose (40+ 0.2 mg / Kg diet) leads to higher value of serum antioxidant activity by about 33 % of control diet. The results indicated that the diet contained 0.2mg organic Se, 60 mg organic Mn and the mixture between 40 mg organic Mn+0.2mg organic Se /kg diet improved egg production performance, profile lipids, antioxidant activity in eggs and blood serum as well as economic efficiency of egg production.

journal of animal and poultry production

Effect of Dietary Organic Selenium and Manganese Supplementation on Productive Performance of Local Laying Hens Fed Diet Contained Soybean Oil as a Source of Essential Fatty Acids.