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Effect of some nutritional and environmental factors on growth and cellulase production by Bacillus alcalophilus S39 and Bacillus amyloliquefaciens C23 was investigated. Results indicated that 1% carboxymethylcellulose (CMC) and 0.7% yeast extract were most effective as the carbon and nitrogen sources respectively. Initial pH 7 and 3% inoculum size (3.5-4.3 ×10 6 CFU/ml) found to be optimal for growth and cellulase production. Incubation temperature at 30oC and 45oC achieved the highest activity of cellulase for Bacillus alcalophilus S39 and Bacillus amyloliquefaciens C23 respectively, and the suitable shaking rate was 150 and 200 rpm.

NUTRITIONAL AND ENVIRONMENTAL FACTORS AFFECTING CELLULASES ACTIVITY PRODUCED BY HIGH POTENT CELLULOLYTIC BACILLI