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An experimental study was carried out to compare various noncommercial methods blood culture for isolation of Brucella melitensis from fresh bovine blood samples that has been artificially inoculated with 100 microorganisms per ml of blood. The methods compared included the Castaňeda blood culture (BC), leukocyte lysis and direct plating on agar (LLD), leukocyte lysis and filtration (LLF), Ficoll centrifugation (FC), Ficoll centrifugation and filtration (FCF), Ficoll centrifugation and leukocyte lysis (FLLD), and Ficoll centrifugation and leukocyte lysis filtration (FLLF). Results with the LLD technique showed that B. melitensis was isolated in 48 h, and its recovery rate was 57% (calculated from the number of CFU recovered per milliliter versus the number inoculated). The isolation times for other blood culture techniques were between 24-216 h. The LLD technique for isolation B. melitensis is faster and cheaper than the other methods tested.

journal of agricultural chemistry and biotechnology

COMPARISION OF DIFFERENT BOVINE BLOOD CULTURE TECHNIQE FOR BACTERIOLOGICAL ISOLATION OF Brucella melitensis مقارنة طرق مختلفة لإستنبات دم الأبقار للعزل البکتیری للبروسیلا الضأنیة