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A total number of 720 turkey hatching eggs with an average weight of 81± 1g were used to study the effect of injecting the eggs with pyridoxine (vitamin B6) at 10, 17 and 24 days of incubation. Hatching eggs were divided into equal six groups at each injecting time. The 1 group without injection or pierced was served as control 1, the 2 group without injection was pierced in the blunt end and served as control 2, while eggs of the 3 group was injected into amniotic sac through the air sac with 0.2 ml saline solution. The eggs of the 4, 5 and 6 groups were injected as group 3 with 400, 600 and 800 μg pyridoxine, respectively, dissolved in 0.2 ml saline solution. The results obtained could be summarized as follows: Embryonic mortality percentage (EM) as well as hatchability percentage (H) and chick weight at hatch (CWH) showed significant (P≤0.05) differences among injection times of embryonic development (ED). The best EM, H and CWH values were recorded at the10 ED. Injection of 0.2 ml saline solution with different levels of pyridoxine to the hatching eggs, significantly (P≤0.05) decrease the EM and significantly (P≤0.05) improved both H and CWH. The best value of Albumin (A)/globulin (G) ratio was at the 17 and 24 ED compared to 10 ED. The best values of plasma total protein (PTP), globulin (G) and A/G ratio were recorded by eggs injected with the lowest level of pyridoxine (400μg). Moreover, the best value of A/G ratio was recorded by control group at the 24 ED. The best value of plasma cholesterol level was recorded for eggs injected the highest level of pyridoxine (800μg) at the 10 ED. Plasma concentrations of thyroid hormones were significantly increased (P≤0.05) by increasing pyridoxine injection. On the other hand, insignificant differences were noted in plasma thyroid hormones concentrations due to periods of ED. The highest values of T3 hormone were recorded by eggs injected the highest level of pyridoxine at both the 24 and 17 ED, respectively. While the highest values of T4 hormone were recorded by eggs injected the highest level of pyridoxine at both the 17 and 10 ED, respectively. In conclusion, in-ovo pyridoxine injection with 800μg to turkey eggs at 10 and 17 day of incubation is a practical and beneficial procedure to improve the hatchability traits.

EFFECT OF IN-OVO INJECTION OF PYRIDOXINE ON HATCHABILITY AND PHYSIOLOGICAL RESPONSE OF HATCHED TURKEY POULTS