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A total number of 189 local Sinai laying hens (162 female + 27 males), 25weeks-old, were equally divided into nine dietary treatments to investigate the effect of dietary different sources of trans-anethol (anise seeds or oil) included three levels of trans-anethol (0, 140 mg trans/anethol from adding 4g anise seeds and 140 mg trans-anethole from adding 180mg anise oil/kg diet) and three levels of cholecalciferol (vitamin D3) in 3×3 factorial design on productive and reproductive performance during the laying period (25-40 weeks of age). The dietary levels of trans-anethole and vitamin D3 included 3x3 factorial design (0, 4g anise seeds and 180 mg anise oil/kg diet, each contained 2000, 2750 and 3500 IU vitamin D3/kg diet). The results show that hens fed diet 180 mg anise oil/ kg diet alone or with 3500 IU vitamin D3/ kg diet had the highest record of egg production and significantly exceeded basal diet and other treatments. Also, the high level of vitamin D3 (3500 IU/ kg diet) produced significantly higher egg production than basal diet (2000 IU/kg diet). The best value of feed conversion was observed as a result of the diet with 180 mg anise oil/ kg diet, 3500 IU vitamin D3 and the interaction between the same treatments compared to the basal diet. Hatchability % and chick weight at hatch significantly increased due to addition of 4 g anise seed /kg diet compared to the basal diet. Moreover, addition of 2750 IU vitamin D3 /kg diet resulted in significantly higher hatchability of fertile eggs and chick weight at hatch than by basal diet. Calcium content in blood serum (mg/ dl) significantly decreased for hens fed diet supplemented with 180 mg anise oil/ kg diet compared to the basal diet. Interaction between 180 mg anise oil and 3500 IU vitamin D3 /kg diet resulted in a significant increase in total protein of serum. A significant decreased was observed in egg shell calcium content and tibia phosphorus (mg/g) due to addition of 4g anise seed and 180 anise oil/ kg diet compared to the control diet. In this respect, results revealed that the calcium content of egg shell was significantly decreased due to interactions between most dietary treatments. The results revealed an important role of adding 180 mg anise oil + 3500 IU vitamin D3/kg in Sinai laying hens diets for maximizing the productive performance and economic efficiency, and the addition of 4 g anise seed powder + 2750 or 3500 IU vitamin D3 to maximize hatchability % during the period 25-40 weeks of age.

EFFECT OF DIETARY CHOLECALCIFEROL AND ANETHOLE SOURCE ON PRODUCTIVE AND REPRODUCTIVE PERFORMANCE OF LOCAL LAYING HENS 1-FROM 25 TO 40 WEEKS OF AGE