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SEVERAL factors that influence the preparation of 186Re-Cefixime such as the amount of Cefixime and stannous chloride, pH, reaction time and reaction temperature were studied to optimize the labeling conditions to obtain the highest radiochemical yield of 186Re-Cefixime. The radiochemical purity of Rhenium-186 was determined by paper chromatography, while the radiochemical yield and purity of 186Re-Cefixime were determined by electrophoresis and high-performance liquid chromatography (HPLC). The maximum radiochemical yield of 186Re-Cefixime was obtained (96±2.8%) using 2mg Cefixime, 0.3mg carrier added 186Re and 0.5mg stannous chloride at pH 5.5, within 30min at room temperature. The bio-distribution was carried out on three types of mice (normal, sterile infected and bacterial infected). The results show that 186Re-Cefixime is more concentrated in the bacterially infected muscle (septic inflammation) than in the sterile infected muscle (aseptic inflammation). Therefore, 186Re-Cefixime could be used to differentiate between septic and aseptic inflammation.

Preparation of 186Re-Cefixime as a Potential Diagnostic and Therapeutic Agent for Bacterial Infection