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Two DNA molecular marker systems, RAPD and ISSR were used to assess genetic diversity among nine tomatos (Lycopersicon esculentum L.), one tomatille (Physalis philadelphica) and one cherry tomato (Solanum lycopersicum var. cerasiforme) accessions, collected from different regions in Egypt. Accurate and unambiguous identification of these accessions is essential for germplasm preservation and use. Genomic DNA from the 11 accessions was screened with 18 ISSR primers and nine RAPD primers. A total of 293 and 180 clear fragments were amplified by ISSR and RAPD, respectively. On the other hand, unique positive markers were detected for ‘Tomatillo’ and for ‘Super marmande’, by 4 and 3 RAPD primers, respectively. Moreover, 18 primers of ISSR produced unique positive markers for Tomatille and Castle Rock, respectively. The ISSR technology proved useful in describing genetic diversity among tomato accessions and studies the phylogenetic relationships between cultivars. Cluster analysis using the UPGMA method placed all tomato accessions and cultivars into a single group, while the Tomatille and cherry tomato accessions were placed in a second group.

ASSESSMENT OF GENETIC DIVERSITY OF TOMATO (Lycopersicon esculentum L.) GERMPLASM USING MOLECULAR MARKERS (RAPD AND ISSR)