English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The air dried leaves of Laurus nobilis cultivated in Egypt were extracted by petroleum ether followed by methylene chloride then ethyl acetate and finally methanol. The preliminary cytotoxic screening of these extracts against Hep-G2 using vinblastine sulfate (IC50= 2.93 μg/ml) as standard showed that petroleum ether, methylene chloride and ethyl acetate extracts exhibited strong cytotoxic activity (IC50= 10.6, 5.96 and 3.80 μg/ml, respectively) while the methanolic extract showed moderate activity (IC50= 23.2 μg/ml). Bio-guided chromatographic isolation of L. nobilis extracts resulted in isolation of five compounds identified as 1-tricosanol (1), reynosin (2), protocatechuic acid (3), vincetoxicoside B (4) and vitexin (5). The chemical structures have been established on the basis of physical, chemical and spectroscopic methods (UV, IR, MS, H-NMR and C-NMRand HSQC) in addition to comparison with literature data and /or authentic samples. Reynosin (2) showed the most potent cytotoxic activity against Hep-G2 (IC50= 4.98 μg/ml) among other isolated compounds, while vitexin (5) showed the lowest activity (IC50= 219 μg/ml). Other compounds showed moderate to weak activity. The isolated compounds were docked to caspase3 to reveal their possible cytotoxic mechanism of action on the enzymatic level. Protein fraction obtained after 0.5 hr hydrolysis with papain showed moderate cytotoxic activity (IC50= 200 μg/ml) and that obtained after 3.5 hr hydrolysis exhibited strong antioxidant action (45 % inhibition at a concentration of 200 μg/ml using DPPH assay).

Bioguided isolation and in-silico analysis of Hep-G2 cytotoxic constituents from Laurus nobilis Linn. cultivated in Egypt