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This study aimed to investigate the relationship between the protein profile of seminal plasma and fertility rate of some subtropical sheep breeds .By using artificial vagina three ejaculates were collected from ten mature rams(Ossimi (3), crosses of Ossimi × Finnish Landrace and Suffolk (3), and Assaf (4) breeds).Semen characteristics e.g. (ejaculate volume, semen density, mass motility, individual motility (%), live sperm (%), abnormalities (%) and sperm concentration were determined after collection. Seminal plasma proteins were identified by one dimensional polyacrylamide gel electrophoresis (SDS-PAGE) method. Semen density and sperm concentration were significantly (p<0.05) higher in both Assaf and Crossbreds of Ossimi. Abnormal sperm percentage revealed a significant increase in Ossimi semen (9.80%) compared to its crossbreds and Assaf semen (7.83 and 6.97%, respectively).A total of 14 protein bands were visualized in ram seminal plasma samples. Protein bands 78-85 and 97-107 KDa were present in all studied breeds. However, the protein band of 40 KDa showed an increase in the percent of appearance in Ossimi crossbreds and Assaf (100%) compared to Ossimi breed (33%).It is clear that Assaf rams proved a potential capability for their breeding ability under Egyptian conditions. Therefore, it may be recommended to incorporate this breed in breeding programs of sheep in Egypt.

CHARACTERISTICS OF SEMINAL PLASMA PROTEINS IN RELATION TO FERTILITY OF SHEEP UNDER EGYPTIAN CONDITIONS