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Forty six pure Holstein heifers were assigned randomly to two synchronization treatments Select Synch (n = 21) and MGA 7-11 Synch (n = 25). The MGA 7-11 Synch heifers were fed MGA (0.5 mg/h/day) for 7 days concluded with PGF2α injection. Four days later, both groups (Select Synch and MGA 7-11Synch) were administered GnRH followed with PGF2α on day 0. Heifers were inseminated with frozen semen according to the AM/PM rule. Within 24-48h peak response period after PGF2α injection more (81%, 17/21) Select Synch heifers displayed estrus compared to MGA 7-11 Synch (68.1%, 17/25). However, overall percentages of heifers detected in estrus were not different between Select Synch (95.2%, 20/21) and MGA 7-11 Synch (84%, 21/25) heifers. Select Synch heifers had empirically improved (P=0.2) conception rate to first artificial insemination (AI, 75%, 15/20) compared to MGA 7-11 Synch heifers (57%, 12/21), while overall pregnancy rates were 95.2 and 96%. The total number of follicles (TNF) on both ovaries was not different between the Select Synch (2.4±0.3) and MGA 7-11 Synch (2.2±0.3) heifers. Likewise, the overall diameters of largest follicles were not affected by treatments. However, Select Synch heifers had larger (P<0.05) follicles (11.4±0.9mm) than the MGA 7-11 Synch (9.2±0.6 mm) heifers on day -7. Percentages of induced CLs on days 0 (62 vs. 76%) and 10 (76.2 vs. 80%) after PGF2α injection were not different between Select Synch and MGA 7-11 Synch heifers. Similarly, diameters of induced CLs on days 0 (19.3+2.9 vs. 18.3+2.5 mm) and 10 (19+2 vs. 21.3+1.6 mm) were not different. Progesterone concentrations were greater (P<0.01) on day -7 in the Select Synch (7.1±1ng/ml) compared to the MGA 7-11 Synch (2.5±0.7ng/ml). Short-term feeding of MGA (MGA 7-11Synch) to dairy heifers produced acceptable overall pregnancy rates while Select synch treatment tightened the synchrony for estrus compared to MGA 7-11 Synch. Further research is needed to improve estrual responses and synchrony after short-term of MGA feeding to dairy heifers.

SHORT-TERM FEEDING OF MELENGESTROL ACETATE PLUS GnRH AND PGF2α TO ENHANCE REPRODUCTIVE EFFICIENCY OF REPLACEMENT DAIRY HEIFERS