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Objective: To evaluate the role of anti-annexin V antibodies (aANX) and anticardiolipin antibodies (ACA) as risk factors and predictors for in vitro fertilization-embryo transfer (IVF or ICSI) outcome. Design: Prospective study, Setting: IVF unit, Int. Islamic center for population studies and researches, Al-Azhar University, and Health centers in 10 of Ramadan city. Subjects and Methods: Were classified into 3 groups:Group I: 30 women had one or more failed IVFJET despite good morphological quality of the transferred embryos. Group II: 30 non-pregnant women had previous history of live births. Group III: 30 pregnant women in the first trimester as controls previously delivered normally and no previous history of pregnancy loss. All were submitted to: 1) Detailed history to eliminate any case with renal, hepatic, or metabolic diseases. 2) Complete medical examination and laboratory investigations to exclude autoimmune disease, infection. hypertension, D.M., or other endocrinal disease. 3) aSerum antiannexin V antibodies were measured by Eliza. b-Serum anticardiolipin antibodies were measured by Eliza. Results: 1) Serum antiannexin V IgG and serum anticardiolipin IgG were increased significantly in group I compared to the other groups. 2) There was no significant difference between group II&III for both parameters of antiannexin V IgG & anticardiolipin IgG with lower level in group III than group II. Conclusion: The antiannexin V IgG & anticardiolipin IgG both can be used as predictors for IVF-ET success.

OUTCOME OF IN-VITRO FERTILIZATION AND THE AUTO ANTIBODIES AGAINST ANNEXIN V AND CARDIOLIPIN