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Alveolar ridge preservation techniques and biomaterials were extensively used in the past few years. The rate of new bone formation is the concept in healing sockets. The faster the rate the lesser the alveolar bone resorption. Resorption is fastened in the first three months after extraction. Wide range of biomaterials were available, the choice of an ideal material was confusing.Objective: Evaluate the effectiveness of both Moringa Oleifera (MO) leaf powder and extract mixture locally and MO leaf extract repeated injections in fresh extraction sockets of dog. Moreover, identifying bioactive phenolic compounds in dried leaves.Materials and Methods: Eight mongrel dogs were included in this study; two animals were used as control. Six test animals were divided into two groups according to biopsy time (3 and 5 weeks). Each of these groups includes three animals. All animals were subjected to extraction of the third premolar bilaterally, both sides of the test animals (the six) were treated with Moringa Oleifera leaf powder (0.7 gm) and water extract (1ml) mixture. The right side only was subjected to repeated injections of Moringa Oleifera leaf water extract (½ ml) for two times (1st week and 2nd week postoperatively for all groups). Trephine bur was used to take biopsy for the purpose of histopathologic examination by H&E and Masson Trichrome stain to quantify new bone formation, and number of inflammatory cells.Results: Bone area percent showed the highest mean value in the right side at 3 weeks and 5 weeks. ANOVA test revealed no significant difference between groups (P=0.43) at 3 weeks, while the difference was significant at 5 weeks (P=0.00). Mean inflammatory cells count were significantly higher mean value in control group at 3 weeks and 5 weeks (P=0.00). Conclusion: Two injections of moringa oleifera aqueous extract of 11.7% concentration improved bone area present significantly at 5 weeks and decreased inflammatory cells at all test periods.

Improvements of alveolar bone healing using Moringa oleifera leaf powder and extract biomimetic composite: an experimental study in dogs