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This study was conducted to evaluate the efficacy of the bio-insecticide Bacillus thuringiensis (Dipel 2x ® 6.4 % WP) against, 1 st and 2 nd instar Larvae of Sesamia cretica compared with methomyl (Lannate 90 % SP). After 48 hours from treatment, The LC50 value of B. thuringiensis for 1 st instar and 2 nd larvae were 0.00526 and 0.7 gm, respectively. While the LC50 of methomyl after 24h. for 1 st instar and 2 nd larvae were 3394 and 5481 ppm, respectively. After treatment of 1 st and 2 nd instars larvae of S. cretica with LC50 concentrates of B. thuringiensis, the average larval duration of S. cretica were (36.53 & 24.69) and (33.78& 20.31) days for treated and untreated, respectively. In addition, the pupation percentage was (47 & 92 %) and (18 & 84 %) for treated and untreated, respectively. The larval mortality percentage was (53 & 8 %) and (82 & 16 %) for treated and untreated, respectively. The pupal weight for (treated & untreated), S. cretica were (0.1887 & 0.184 gm) and (0.1842 & 0.1601 gm) when it treated as 1 st and 2 nd instars larvae, respectively. Furthermore, the pupal duration were (12.14 & 10) and (10.44 & 9.83 days) for (treated & untreated), when S. cretica treated as 1 st and 2 nd instar larvae, respectively. The total adult emergence for 1 st instar and 2 nd instar larvae were (94 & 100%) and (100 & 100%) for treated and untreated, respectively. On the other hand results indicated that, after treatment of 1 st and 2 nd instar larvae of S. cretica with LC50 concentrates of methomyl, the average larval duration of S. cretica were (31.41 & 24.69) and (33.87& 20.31) days for treated and control, respectively. The pupation percentage was (34 & 92 %) and (28& 84 %) for treated and untreated when S. cretica treated as 1 st and 2 nd instars larvae, respectively. The larval mortality percentages were (66 & 8 %) and (72 & 16 %) for treated & untreated, respectively. The pupal weight for treated and control, were (0.1626 & 0.184 gm) and (0.1994 & 0.1601 gm), respectively. The pupal duration were (12.41 & 10) and (10.83 & 9.83) days for treated and untreated, respectively. The total emergence percentage for 1 st instar larvae were (100 & 100 %) and (85.7& 100%) for 2 nd instar larvae for each (treated & untreated), respectively.

egyptian academic journal of biological sciences. f, toxicology and pest control

Assessments the toxic effects of entomopathogenic bacterium, Bacillus thuringeinsis subsp. kurstaki, and methomyl insecticide on larval instars of the greater sugarcane borer; Sesamia cretica (Lederer)