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The present study aimed to evaluate the biological effect of insect growth regulator flufenoxuron (Cascade) as a chitin synthesis inhibitor against 2 nd and 4 th larval instars of Spodoptera littoralis, to determine its toxicity. Effect of sublethal doses LC25, LC50 and LC90 were used to investigate the enzymatic activities. The tested IGR significantly increased the larval and pupal durations, on the other hand decrease the percentages of pupation, adult emergency, fecundity and fertility of the eggs produced by the adult progeny. The tested compound significantly induced larval mortalities, which were dose dependant. Treatments of the 2 nd and 4 th larval instars with the tested IGR induced some morphogenic abnormalities in larval, larval-pupal and pupal stages, as well as pupal-adult intermediate. Some emerged adults have various degrees of malformations. All the treated larvae as 2 nd instar showed a high sensitivity to the tested IGRs more than 4 th instars. The treated larvae in both 2 nd and 4 th larval instars with the sublethal doses LC25, LC50 and LC90 showed a significant decrease in enzyme activities of acid phosphatase and the nonspecific esterases, α,β esterases at different times intervals post treatments.

egyptian academic journal of biological sciences. f, toxicology and pest control

Effect of Chitin synthesis inhibitors (flufenoxuron) on some biological and biochemical aspects of the cotton leaf worm Spodoptera littoralis Bosid (Lepidoptera: Noctuidae)