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A laboratory study on the 4 th instar larvae of cotton leaf worm. , Spodoptera littoralis (Biosd.) was carried out to detect and quantify some nitrogenous components related to protein metabolism and also to evaluate the secondary effect of the bioinsecticide; Spinetoram. It is the first time to detect albumin and creatinine in this species. Acute and latent effects using three concentrations revealed that the excretory product; uric acid was significantly reduced 6-days post treatment by the highest concentration (6.67ppm). It was 14.15 and 10.94 ug/mg protein for control and treated larvae, respectively. Creatinine which is an end product of phosphocreatine that acts as energy storage in skeletal muscles and other tissues is significantly increased on the 4 th day post larval treatment. The insecticidal concentration range was not critical in most cases. Albumin was significantly reduced at the 4 th day post treatment and this reduction extended significantly to 6 th day for all concentrations.Glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminases (GPT) titres were significantly reduced as compared to control at the same time, the situation that reflects the importance of Spinetoram latent effect. Spinetoram could affect protein metabolism of the cotton leaf worm for energy reserves, metabolic enzymes, protein quality and excretory metabolism point of view.

egyptian academic journal of biological sciences. c, physiology and molecular biology/egyptian academic journal of biological sciences. c, physiology and  molecular biology

Impact of Spinetoram on Some Nitrogenous Components Related to Protein Metabolites in the Cotton Leaf Worm, Spodoptera littoralis (Biosd.)