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Green synthesizednanoparticles have been studied as  newand more effective biopesticides against insects of medical importance and thisfor their eco-friendly aspects. In the current study Moringa oleiferaleaf extract (Mo-LE) was used to synthesis silver nanoparticles (Mo-AgNPs) andzinc oxide nanoparticles (Mo-ZnONPs). Low concentrations of Mo-LE, Mo-AgNPs andMo-ZnONPs showed the larvicidal and pupicidal toxicityagainst Musca domestica. In larvicidal bioassay, the values of LC50were 16.50, 2.03 and 6.41 mg/ml for Mo-LE, Mo-AgNPs andMo-ZnONPs,respectively. In addition, the LC50 was determined against pupae,which were129.77, 9.604 and 17.10 mg/ml for Mo-LE, Mo-AgNPs andMo-ZnONPs,respectively. Moreover, treatment larvae with LC50's of Mo-LE, Mo-AgNPs andMo-ZnONPsproduced a significant reduction (P<0.05) in the fecundity of femaleand a highly significant reduction (P<0.01) in the egg hatchability.The total protein content and activities of nonspecific esterases, acetylcholine esterase, and glutathione S-transferase enzymes were significantly declined afterlarvae had fed on LC50 Mo-AgNPs andMo-ZnONPstreated diets as compared with the control. This study indicates that it isfeasible to use M. oleifera synthesized nanoparticles as effective candidates to develop newer and cheapcontrol tools for immature stages of M. domestica.

Insecticidal Activity of Moringa oleifera Synthesized Silver and Zinc Nanoparticles against the House Fly, Musca domestica L.