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Nematophagous fungi include a group of fungal antagonists to nematodes. The nematophagous fungi have been suggested as promising candidates for biological control of plant parasitic nematodes. Impacts of different pH levels, temperature, light intensity, carbon and nitrogen source were tested on the growth of five nematophagus fungi under in vitro conditions. Three isolates of Trichoderma harzianum T7, T8, and T14, one isolate of Pochonia chlamydosporia var. chlamydosporia and one isolate of Arthrobotrys oligospora were tested. The results of experiment indicated that the growth of isolates of Trichoderma harzianum was maximum in pH range of 7.008.00. The best growth of Pochonia chlamydosporia var. chlamydosporia and Arthrobotrys oligospora was in 4.00-7.00. Effect of temperature differs between range of 15-30°C, for isolates of Trichoderma harzianum was 20-25°C, Pochonia chlamydosporia var. chlamydosporia was 15-20°C, and Arthrobotrys oligospora was 20-25°C. Maximum growth of two isolates of Trichoderma harzianum T7, T8 and Pochonia chlamydosporia var. chlamydosporia was in darkness. Best growth of one isolates of T. harzianum (T7) was in light and maximum growth of Arthrobotrys oligospora and Trichoderma harzianum T14 occurred in 12 h light and 12h dark. The best source of nitrogen for Trichoderma harzianum (T8,T14) was Nitrate potassium, while Nitrate ammonium was the best nitrogen source for (T7).The maximum growth of A. oligospora and P. chlamydosporia occuerd in Nitrate sodium. Between carbon sources Arabinose are suitable for A.oligospora and Trichoderma harzianum (T7).The maximum growth of P. chlamydosporia and T. harzianim (T14) occuerd in the use of fructose. Best growth of T. harzianum (T8) was in Mnitole.

Effect of cultural condition on biomass production of some Nematophagous fungi as biological control agent