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Experiments were designed to investigate the molecular immune response ofSpodoptera littoralis larvae against bacterial infection. In addition, sequence andphylogenetic analyses of the involved gene were studied. Using differential displaytechnique, a partial insect lectin gene (SpliLec) was isolated from bacterial-challengedS. littoralis haemolymph. Five differentially displayed bands were sequenced.Sequence results revealed that a fragment of 640 bp was amplified within the openreading frame (orf) of a lectin gene. This fragment contained the complete 3` end witha poly(A) tail, but it lacks start codon, AUG at its 5` end. Using RACE PCR reaction,5` end was extended and a final reaction was performed to obtain the full length of theSpliLec. Sequence analyses of the data revealed that SpliLec consists of a single orfencoding a deduced polypeptide consisting of a 18-residue signal peptide and a 291-residue mature peptide. SpliLec sequence contained two CRDs: short form CRD1 andlong form CRD2 stabilized by two and three highly conserved disulfide bonds,respectively. SpliLec shares homology with some dipteran lectins suggesting possiblecommon ancestor. These results suggested an important role of the SpliLec gene incell adhesion and non-self recognition. It may cooperate with other AMPs inclearance of invaders of Spodoptera littoralis.

egyptian academic journal of biological sciences. a, entomology

Molecular Characterization, Full Length Isolation and Phylogenetic Analysis of C-type Lectin Gene from Bacterial-challenged Cotton Leafworm, Spodoptera littoralis