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Male Drosophila melanogaster, like males of many other animal species, male age and temperature affected the sperm number. Here we use Drosophila melanogaster males of different ages and decreasing temperature on the sperm number, to investigate the role of aging and temperature affected to sperm transferred to female during mating. The male ages were used to show that while maximize sperm number in young (2-3 days)and middle age (27-28 days) was contributed but mean value of sperm number between fifty replicate was different and also the results of one way ANOVA were significant between three age classes. Sperms after mating soon counted from female reproductive system, the results are shown, mean value of sperms when males exposed to cold temperature (15°c) in young and middle aged, sperm number was around 4000, and old male aged (952-53 days) around 2000, these results demonstrate that viability of sperm produce in males after 27- 28 days, will be reduced more than young to middle aged.

Comparison of sperm viability in three male age of Drosophila melanogaster exposure of cold temperature