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The use of vaccines in the prevention and control of infectious diseases in the horse is a widely accepted practice. A combined inactivated EIV, EHV-1 and tetanus toxoid vaccine adjuvanted with alhydrogel and saponin was evaluated in horses. Two groups of horses, group A was inoculated with two doses of the combined vaccine one month apart and revaccinated at 6 months and group B was kept as control. The immune response of vaccinated horses against EI, EHV-1 and tetanus was evaluated by measuring Haemagglutination Inhibition (HI), Enzyme linked immunosorbent assay (ELISA), Complement Fixation (CF) and toxin neutralizing antibody titers (TN). Vaccinated horses had high antibody titers, above the level for clinical protection against influenza and tetanus until 12 months and 6 months for EHV-1 after the third vaccination. A vaccination scheme consisting of three doses with combined inactivated EI, EHV-1 and TT vaccine adjuvanted with saponin and alhydrogel, two doses at one-month interval and Revaccination after 6 months, Followed by alternating vaccinations with monovalent inactivated EHV-1vaccine and combined inactivated EIEHV& TT vaccine every 6 months is proposed to maintain maximal protection against equine influenza and herpesvirus and tetanus infections.

Field Evaluation of the prepared combined equine influenza, equine herpesvirus-1 and tetanus toxoid vaccine in horses