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Meat species specification is a vital field to ensure the food safety to the consumers which corresponds to the laws related to meat and meat products. The adulteration of inferior quality meat into superior quality one is a common practice all over the world. In this study, 30 beef product samples including minced beef, luncheon and sausage (10 samples each) were collected from different markets in Cairo and Giza Governorate, transferred to the laboratory for detection of adulteration of beef with pig, equine and dog meat using Simple and reliable multiplex-polymerase chain reaction (multiplex-PCR) for the partial-length of cytochrome b (cyt b) gene of mitochondrial DNA (mtDNA) which indicated the successful detection of little as 0.05 ng % adulteration of meat products. Multiplex PCR can also be applied to detect authentication with equal efficiency to fresh, cooked or putrefied mixed meat samples. The obtained results revealed that one examined sample (10%) of minced beef was intermixed with equine meat only, while two samples (20%) of beef sausage were adulterated with addition of equine meat only. Moreover, the examined luncheon samples were free from adulteration with either pork or equine meat. All examined samples were found to be free from adulteration with dog meat. The bad effects of fraud and adulteration of meat products was discussed.

Molecular assay using PCR based technology to identify fraud and adulteration of some meat products