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A total 100 samples of liver and intestine from broilers (apparently diseased) from 13 poultry farms were analyzed to detect the incidence of C. perfringes. The samples were examined bacteriologically by cultivating on CMC and blood agar, typical colonies were identified and biochemically confirmed. The total number of positive samples was 46 (46%). C. perfringens showed very high sensitivity to enrofloxacin, high sensitivity to amoxicillin and cefoperazone, but resistant for mupirocin and azteronam. By making artificial infection for mice, the mice died after 24 hours and by examination histopathologicaly, it showed liver degeneration and desquamation of epithelial cells of intestine. Multiplex polymerase chain reaction method (PCR) was amplified for toxin genotyping by using primer for clostridium perfringes alpha toxin which amplified at 402 bp.

Rapid methods of diagnosis of Clostridium in broilers in Sharkia governorate