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This study was aimed to evaluate the effect of dietary supplementation of prebiotic (Bio-Mos®, mannoligosacchride), probiotic (Bio-Plus® 2B, Bacillus subtilis and Bacillus licheniformis) and their mixture on growth, biochemical parameters and immune-hematological responses of rabbits. Sixty four New Zealand White male rabbits were divided into 2 equal groups. The 1st group was uninfected and subdivided into 4 subgroups. The 1st subgroup fed basal diet (Control), the 2nd, 3rd and 4th subgroups fed on basal diet supplemented with 1 g Bio-Mos, 0.4 g Bio-Plus and 1g Bio-MOS + 0.4 g Bio-Plus / kg, respectively for eight weeks. The 2nd group was similar to the 1st group but experimentally infected with Pasteurella multocida. The results in 1st group showed significant increase (P<0.01) in body weight gain, phagocytic activity (PA), phagocytic index (PI) and total leukocytic counts (TLC) when compared with control group 1.1. In addition, there was significant decrease in serum total cholesterol, triglycerides and glucose when compared with control group 1.1. In 2nd group , the results showed significant increase (P<0.05) in body weight gain, (P˂0.001) in phagocytic activity and phagocytic index, RBCS count, PCV, Hb concentration, and number of lymphocytes while TLC and number of heterophils showed significant decrease(P<0.001) when compared with control group 2.1 .Also there was significant decrease (P<0.05) in food conversion ratio (FCR), (P<0.01) in total cholesterol and creatinine and (P<0.001) in number of heterophils, triglycerides, glucose, alanine amino transferase (ALT), aspartate amino transferase (AST) and urea in all infected groups fed experimental diets compared with control group 2.1.Supplementing the diet with Bio-Mos, Bio-Plus or their mixture decreased the mortality and improved the adverse clinical signs and post mortem lesions in all experimentally infected groups compared with infected untreated control group. Our results indicate that rabbits received mixture of pre and probiotic groups 1.4 and 2.4 recorded the highest value of daily weight gain, PA, PI, TLC and lymphocytes number and recorded the lowest FCR followed by rabbits received probiotic. Dietary supplementation of prebiotic and probiotic and their mixture improves cell-mediated immune response, liver and kidney functions , decreased the mortality and improved the adverse clinical signs and post mortem lesions in in rabbits experimentally infected with P. multocida.

Effect of Prebiotic and Probiotic on Growth, Immuno-hematological responses and Biochemical Parameters of infected rabbits with Pasteurella multocida