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Inactivated tissue culture adapted Rift valley fever (RVF) virus vaccines were prepared using Montanide oil ISA 61 VG, Montanide oil ISA 201 VG and aluminum hydroxide gel as adjuvants. The prepared vaccines were sterile and safe inducing no systemic or local clinical signs in sheep. Comparative evaluation of experimentally prepared vaccines in sheep after a single dose indicated that the oily prepared vaccines stimulated the humeral immune response as estimated by SNT and ELISA compared with aluminum hydroxide gel vaccine. Protective serum antibody titers against in Montanide oil ISA 201 VG adjuvenated RVF vaccine appeared in protective level at 1st week post vaccination but it appeared as protective in Montanide oil ISA 61 VG adjuvenated RVF vaccine at 2nd week and in aluminum hydroxide gel adjuvenated RVF vaccine at 2nd week. These protective titers persisted till 12th month in the new two oily vaccines and till the 6th month for Aluminum hydroxide gel vaccine. Both of SNT and ELISA indicated that among the used adjuvants Montanide oil ISA 61 VG and Montanide oil ISA 201 VG was preferable.

benha veterinary medical journal

Comparative evaluation of different inactivated Rift valley fever virus vaccine adjuvanted with montanide oil ISA 61 VG, montanide oil ISA 201 VG, and aluminum hydroxide gel